NEW PHYSIOLOGICALLY ACTIVE SUBSTANCE, ITS PRODUCTION AND USE

摘要

PROBLEM TO BE SOLVED: To obtain a new physiologically active substance that comprises a ligand polypeptide of a G-protein conjugate type receptor protein having a specific amino acid sequence and is useful in development of medicines such as pituitary function controller, central function controller or pancreatic function controller. SOLUTION: This substance comprises a new ligand polypeptide of G-protein conjugate type receptor protein bearing an amino acid sequence (substantially) identical to the formula (its amide, ester or salt) and is useful for the development of receptor-linking assay system using a recombinant type receptor protein expression system and the development of therapeutic and preventing agents for a variety of diseases and the development of medicines such as pituitary function controller, central function controller or pancreatic function controller by screening of the agonists or antagonists of the G-protein conjugate type receptor using the receptor-linking assay system. This physiologically active substance is prepared by cloning human pituitary cDNA through the PCR technique using a DNA of a partial base sequence of the pituitary cDNA and expressing the resultant gene in the host cells.