| 摘要 |
<p>The invention concerns a complex between at least a (negatively charged) nucleic acid and at least a positively charged polymeric conjugate, the bond between the nucleic acid and the polymeric conjugate being electrostatic in nature, the polymeric conjugate containing a polymer formed by monomer units bearing free NH3+ functions, and being such that: the free NH¿3?+ functions of said monomer units are substituted in a ratio of at least 10 % by residues causing in weak acid medium destabilisation of cell membranes, in particular the endocytosis vesicle membrane, and/or endosomes; said residues having further the following properties: they comprise a functional group for being fixed to said polymer, they are not active as recognition signal identified by a cell membrane receptor, they can comprise at least one free NH¿3?+ function; said uncharged residues having further the following properties: they comprise at least a hydroxyl group, they are not active as recognition signal identified by a cell membrane receptor, the hydroxyl groups of said uncharged residues being capable of being substituted by at least a molecule which constitutes a recognition signal identified by a cell membrane receptor, with reservation that the whole set of free NH¿3?+ functions is at least 30 % of the number of monomer units of the polymeric network of said polymeric conjugate.</p> |
