发明名称 |
Biomolecular optical sensors |
摘要 |
The invention provides protein-based sensors which report changes at a cell surface membrane. These sensors comprise an endogenous cell surface protein having a post-translationally generated luminescer at a predetermined residue. In operation, the protein adopts one of a plurality of different interconvertable signal-dependent conformations, whereunder the luminescer provides corresponding different luminescence. The cells comprise transgenes encoding the protein sensor which is translated from such transgenes by the host cell's machinery with natural amino acids and expressed on the cell surface. After surface expression, the cell is contacted with a luminescer generating reagent which generates a luminescer at a predetermined residue of the protein. A wide variety of luminescers and chemistries for generating the selected luminescer at the target residue of the sensor protein may be used.
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申请公布号 |
US5756351(A) |
申请公布日期 |
1998.05.26 |
申请号 |
US19970783377 |
申请日期 |
1997.01.13 |
申请人 |
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA |
发明人 |
ISACOFF, EHUD Y.;MANNUZZU, LIDIA M.;MORONNE, MARIO M. |
分类号 |
C07K14/705;G01N33/543;(IPC1-7):C12N5/00;C12N1/20;C12N3/00;C12N1/14 |
主分类号 |
C07K14/705 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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