| 摘要 |
<p>The present invention relates to a method of assessing the effect of 'anti-platelet' agents, which affect the activation state of platelets, by monitoring levels of membrane bound and/or soluble P-selectin in a sample from an individual. In one embodiment, levels of P-selectin are monitored in a sample containing platelets (e.g., a blood sample, platelet rich plasma (PRP)) using an assay for membrane bound P-selectin. In another embodiment, levels of P-selectin are measured in a sample from which platelets have been removed (e.g., platelet poor plasma (PPP), serum), using an assay for soluble P-selectin. Another aspect of the invention includes measuring both membrane bound and soluble P-selectin to monitor platelet activation for patients who are receiving anti-platelet therapy. The method is useful for monitoring the effect of anti-platelet therapy on platelet activation state as reflected in membrane bound and/or soluble P-selectin levels. Anti-platelet therapy can be adjusted accordingly to maintain a desired level of platelet activation. In another aspect, the invention relates to a method of assessing the effect of a vascular intervention, and of coronary artery interventions in particular, on platelet activation state comprising determining the level of soluble P-selectin in a sample. Anti-platelet therapy can be initiated or adjusted in order to achieve a basal activation state, or other desired platelet activation state.</p> |
