发明名称 |
Method of cell surface activation and inhibition |
摘要 |
Disclosed are a peptide and its use in a method of screening test compounds as potential inhibitors of matrix metalloproteinases. The peptide consists of residues Trp 574 to Asp 656 in the TIMP-2 binding site of the C-terminal domain of gelatinase-A as shown by SEQ ID NO:19. The method comprises determining the inhibitory effect of a test compound in a competitive inhibition assay with said peptide in which a Ki/Kd=>1 is deemed an inhibitory effect of said test compound, and in which Ki is the inhibitor constant of said test compound and Kd is the dissociation constant of said peptide. |
申请公布号 |
WO9812309(A3) |
申请公布日期 |
1998.05.22 |
申请号 |
WO1997US16216 |
申请日期 |
1997.09.15 |
申请人 |
WASHINGTON UNIVERSITY;GOLDBERG, GREGORY, I. |
发明人 |
GOLDBERG, GREGORY, I. |
分类号 |
G01N33/50;C07K14/435;C07K14/81;C12N9/64;C12Q1/37;G01N33/15 |
主分类号 |
G01N33/50 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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