RECEPTOR BINDING ASSAY, APPROPRIATE RECOMBINANT FUSION RECEPTOR FOR SAID ASSAY, VECTOR FOR ITS PRODUCTION AND REAGENT KIT FOR IMPLEMENTING THE RECEPTOR BINDING ASSAY

摘要

Receptor binding assay to determine TSH receptor autoantibodies in a biological sample, in which a preparation of a recombinant TSH fusion receptor as TSH receptor preparation is used, which is extended by one peptide radical as compared to a functional TSH receptor, wherein said peptide radical (i) has a label or can be selectively labelled and/or (ii) can be immobilized or is immovable by binding with a selective binding partner, for instance a monoclonal antibody, a metal-chelate resin or a biotin-binding protein without significantly impairing the required functionality of the TSH receptor for the receptor binding assay. Further, a recombinant TSH fusion receptor which has the aminoacid sequence of a functional human TSH receptor, which is extended by one peptide radical with the properties cited supra, specially one which has been produced by using a vaccinia virus/HeLa cells expression system. 00000