| 摘要 |
PROBLEM TO BE SOLVED: To obtain a human Mad-associating protein gene having an analogue with a gene of a bacterium, a yeast, a nematode, a mouse, a human, etc., in the human gene included in Escherichia coil clone containing various human genes, useful for elucidation of morbid state, diagnosis, treatment, etc., of genepathy, cancer, etc. SOLUTION: This human Mad-associating protein gene is the new one containing a base sequence coding an amino acid sequence of formulas I and II. The new gene having an analogue with a gene of a bacterium, a yeast, a nematode, a mouse, etc., in the human gene is found by synthesizing cDNA from mDNA extracted from human fetal encephalon, adult blood vessel and placenta, forming Escherichia coil clone containing various kinds of human genes by transforming the cDNA using a library and by registering the clone, performing elongation by a deoxy terminator method using a cDNA obtained by culturing the each of clones thereof as templates, and determining a sequence of about 400 based from 5' termianl of the human gene included in the registered each clone. The gene is produced by performing an immunologic screening, etc., from the human cDNA library. |
