NOVEL HIGH-AFFINITY SALICYLIC ACID-BINDING PROTEIN

摘要

A high-affinity salicylic acid-binding protein (SABP2) derivable from tobacco and Arabidopsis is disclosed. The tobacco protein has a molecular weight of approximately 25 kDa and reversibly binds SA with an apparent Kd of approximately 90 nM and a Bmax of 10 fmol/mg protein. The SABP2 of the invention may be used to identify analogues of SA. Analogues so identified may be used in plants to augment disease-resistance response pathways or other SA-sensitive processes in which SA plays a role. Possible examples include flowering and alternative respiration. The SABP2 of the invention may also be used to identify and clone a gene or cDNA that encodes it, which then may be used to generate transgenic plants having altered SABP2 levels.