Methods for labeling a material are disclosed. The methods comprise combining with the material (a) a photosensitizer capable upon irradiation of generating singlet oxygen and (b) a chemiluminescent compound capable of being activated by singlet oxygen wherein the photosensitizer and the chemiluminescent compound are incorporated in a particulate matrix or a non-particulate solid matrix. The particulate matrix can be solid or fluid. The methods allow for generating delayed luminescence, which can be realized upon irradiation of the matrix. The methods have application to the determination of an analyte in a medium suspected of containing the analyte. One method comprises subjecting a medium suspected of containing an analyte to conditions under which a complex of specific binding pair (sbp) members is formed in relation to the presence of the analyte and determining whether the sbp member complex has formed by employing as a label a single composition having both chemiluminescent and photosensitizer properties. Upon activation of the photosensitizer property singlet oxygen is generated and activates the chemiluminescent property. Compositions and kits are also disclosed.
申请公布号
DE69316757(T2)
申请公布日期
1998.05.07
申请号
DE1993616757T
申请日期
1993.07.30
申请人
BEHRINGWERKE AG, 35041 MARBURG, DE
发明人
PEASE, JOHN, S., LOS ALTOS, CA 94022, US;KIRAKOSSIAN, HRAIR, SAN JOSE, CA 95129, US;WAGNER, DANIEL, B., SUNNYVALE, CA 94087, US;ULLMAN, EDWIN, F., ATHERTON, CA 94025, US