发明名称 |
ASSAY USING MARKED MICROBIAL HOST CELL STRAINS |
摘要 |
An assay is provided for determining the presence of contaminating microbial host cells in a culturing vessel containing a microbial host cell strain that utilizes more than one carbohydrate source as a substrate comprising: (a) culturing the microbial host cell strain in the vessel, wherein the strain comprises nucleic acid encoding a polypeptide of interest and is genetically marked so as not to utilize a carbohydrate source as a substrate; (b) plating an isolated sample of culture from step (a) onto culture media supplemented with a carbohydrate source not utilized by the host cell strain as a substrate; (c) incubating the plated cells at a temperature and for a time sufficient for any positive colony to grow to a detectable level; and (d) detecting whether any colonies are growing on the supplemented culture media.
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申请公布号 |
WO9818955(A1) |
申请公布日期 |
1998.05.07 |
申请号 |
WO1997US19473 |
申请日期 |
1997.10.10 |
申请人 |
GENENTECH, INC. |
发明人 |
MCFARLAND, NANCY, C.;SWARTZ, JAMES, R. |
分类号 |
C12Q1/02;C07K14/00;C12N1/21;C12P21/00;C12Q1/04;C12Q1/06;C12R1/19;(IPC1-7):C12Q1/04 |
主分类号 |
C12Q1/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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