PROSTAGLANDIN E2 RECEPTOR, ITS PRODUCTION, DNA CODING THE RECEPTOR, VECTOR COMPRISING THE DNA AND HOST CELL TRANSFORMED BY THE VECTOR

摘要

PROBLEM TO BE SOLVED: To produce a new prostaglandin E2 receptor comprising a polypeptide containing a specific amino acid sequence, having an activities bonding to human prostaglandin E2 (PGE2 ) and transmitting the information and useful for treatment of a disease such as inflammatory caused by excess production of the PGE2 . SOLUTION: This prostaglandin E2 receptor is the new human EP3- V receptor comprising a polypeptide containing an amino acid sequence of the formula and having substantially pure form, or a homologue, a fragment having the sequence or its homologue. The PGE2 receptor is useful for prevention and treatment of a disease, such as inflammatory, caused by excess production of the PGE2 and further for screening of agonist and antagonist active material of the PGE2 , because the PGE2 receptor specifically bonding to the PGE2 . The PGE2 receptor is obtained by extracting RNA from human uterus tissue, etc., forming cDNA by using a reverse transcription enzyme, performing a PCR by using the cDNA as a primer, and screening a gene library by using the obtained cDNA as a probe.