POLYMER ADSORBED IMMUNOLOGICALLY ACTIVE SUBSTANCE IMMOBILIZING STATIONARY PHASE

摘要

PROBLEM TO BE SOLVED: To obtain the subject solid phase useful for clinical diagnosis, capable of suppressing nonspecific adsorption and carrying out measurement excellent in sensitivity and reproducibility, by adsorbing a phosphorylcholine group- containing polymer on a stationary phase prepared by immobilizing an immunologically active substance to a carrier. SOLUTION: A phosphorylcholine group-containing polymer of the formula (R<1> is a 1-10C hydrocarbon group) composed of a polymer obtained by polymerizing a polymerizable component containing 2-methacryloyloxyethyl-2'-(trimethyl ammonio)ethyl phosphate is adsorbed on an immunologically active substance immobilized stationary phase prepared by immobilizing an immunologically active substance (e.g. antibody) to a carrier (e.g. titer plate made of polystyrene) to give the objective polymer absorbing immunologically active substance immobilized stationary phase capable of carrying out measurement excellent in sensitivity and reproducibility while preventing nonspecific adsorption to an immunologically active substance-immobilized stationary phase useful in a two- site method (sandwich measurement) widely used in the field of clinical diagnostic.