发明名称 |
DNA polymerase with proof-reading 3'-5' exonuclease activity Bacillus stearothermophilus |
摘要 |
The invention relates to a thermostable Bacillus stearothermophilus DNA polymerase which is capable of proofreading 3'-5' exonuclease activity during DNA sequencing of a DNA strand, such that the DNA polymerase functions to excise mismatched nucleotides from the 3' terminus of the DNA strand at a faster rate than the rate at which the DNA polymerase functions to remove nucleotides matched correctly with the nucleotides of the template, and which DNA polymerase does not exhibit 5'-3' exonuclease activity. The invention also relates to strains of Bacillus stearothermophilus capable of producing the thermostable DNA polymerase.
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申请公布号 |
US5747298(A) |
申请公布日期 |
1998.05.05 |
申请号 |
US19950544643 |
申请日期 |
1995.10.18 |
申请人 |
SHANGHAI INSTITUTE FOR BIOCHEMISTRY, CHINESE ACADEMY OF SCIENCES |
发明人 |
HONG, GUO FAN;ZHAI, DECEASED, FENG |
分类号 |
C12N9/12;(IPC1-7):C12P19/34;C12N1/20 |
主分类号 |
C12N9/12 |
代理机构 |
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