| 摘要 |
Culture media, having low osmolarity, for establishing and maintaining hormone-secreting cells in long-term culture are disclosed. Cells are derived from tumorous or non-tumorous animal or human tissues including ovary, endometrium, trophoblast, pituitary, thyroid, and pancreas. The cells secrete into the culture media hormones such as estrogens, progestins, follicle-stimulating hormone, luteinizing hormone, human chorionic gonadotrophin, thyroxin, glucagon, and insulin, depending on the tissue of origin of individual cell cultures. The cells proliferate in vitro for up to one year or longer, during which time they retain their hormone-secretion profile.
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