Preperation of purified (poly)peptides

摘要

A method for the modification of (poly)peptides to facilitate their purification comprises the insertion of at least one specific cleavable amino acid (aa) at the end of the (poly)peptide chain during synthesis and protecting any aas within the polypeptide sequence that are the same as the specific aa against cleavage, in order to allow for specific cleavage precisely at the specific inserted cleavable aa(s). Also claimed is a process for the preparation of purified (poly)peptides using the above modification method, the process comprising: (a) synthesising the desired (poly)peptide; (b) adding at least one specific cleavable aa at the end of the (poly)peptide, while protecting the same aa(s) present within the (poly)peptide against cleavage; (c) elongating the (poly)peptide with aa(s) comprising a tag sequence; (d) purifying the elongated (poly)peptide by means of a tag-specific purification method; and (e) removing the tag sequence and the additional aa(s) from the elongated (poly)peptide by means of a cleavage method specific for the additional aa(s) to obtain the purified (poly)peptide.