| 摘要 |
PURPOSE:To execute fluorometry with high accuracy by passing the fluorescence from a specimen which scatters the light irradiated thereon to a lateral side approximately with the advancing direction of said light through a spectral means such as prism or diffraction grating, etc. and detecting the light by a line sensor, etc. CONSTITUTION:The specimen such as hemocyte cells enveloped by a sealing liquid and labeled by a fluorescent agent is passed through the inside of a passage part 2 at the center of a cell 1 in a particle analyzing instrument to be used for a flow sight meter, etc. The fluorescence to be emitted to the lateral side approximately orthogonal with the advancing direction of the irradiated light L is made into a parallel luminous flux by a collimator lens 8. Said flux is divided to continuous frequency components by a spectral prism 9 and the components are condensed by a lens 10 and are made incident to a photoelectric detector 11 such as line sensor. The output thereof is processed by an arithmetic means and the information for analyzing the sample S is obtd. The photometry is thus made possible with high wavelength resolution without requiring an intricate optical system. |
