| 摘要 |
A method by which Indica rice can be efficiently transformed. In this method, immature Indica rice germ cells are transformed by the Agrobacterium method and the resultant transformants are screened with the use of media containing 2,000 to 4,000 mg/l of KNO3, 60 to 200 mg/l of MgSO4, 200 to 600 mg/l of KH2PO4, 100 to 450 mg/l of CaCl2, 200 to 600 mg/l of (NH4)2SO4, 1 to 7 mg/l of H3BO3, 2 to 20 mg/l of MnSO4, 20 to 50 mg/l of EDTA or its salt, 3 to 8 mg/l of Fe, 50 to 200 mg/l of myoinositol, 0.5 to 10 mg/l of 2,4-dichloropheoxyacetic acid, 0.01 to 5 mg/l of cytokinins, 5,000 to 80,000 mg/l of saccharides and gelling agents and having a pH value of 4.5 to 6.5.
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