| 摘要 |
<p>An enzymatic method for producing N-formyl-α-L-aspartyl-L-phenylalanine methyl ester by a condensation reaction between N-formyl-L-aspartic acid and L-phenylalanine methyl ester or D,L-phenylalanine methyl ester, which comprises: supplying an organic phase comprising a water-immiscible solvent having a distribution ratio of N-formyl-α-L-aspartyl-L-phenylalanine methyl ester, DF-APM, as described by following equation (1): DF-APM = PF-APM/1 + 10pH-pKF-APM of 10-2 or more at pH=6.0, and containing N-formyl-L-aspartic acid and L- or D,L-phenylalanine methyl ester, onto an aqueous phase comprising a thermolysin-like metalloprotease; proceeding the condensation reaction in the aqueous phase to produce N-formyl-α-L-aspartyl-L-phenylalanine methyl ester; and extracting the N-formyl-α-L-aspartyl-L-phenylalanine methyl ester thus produced from the aqueous phase to the organic phase.</p> |
