| 摘要 |
<p>The present invention relates to a method for the detection and/or typing of Helicobacter pylori (H.pylori) strains presents in a sample comprising the steps of: (i) if need be releasing, isolating or concentrating the polynucleic acids in the sample; (ii) amplifying the polynucleic acids of relevant target regions of the vacA gene and possibly other virulence determinant genes (VDG), with suitable primer pairs, said primers being generally applicable on different H.pylori strains, allowing to amplify said relevant target regions of the VDG preferentially in compatible amplification conditions; (iii) hybridizing the polynucleic acids obtained in (i) or (ii) with a set of at least two VDG-derived probes, under appropriate hybridization and wash conditions, and with at least one of said probes hybridizing to a conserved region of a VDG of H.pylori, and with at least one of said probes hybridizing to a variable regions of vacA; (iv) detecting the hybrids formed in step (iii); (v) detecting and or typing H.pylori strains present in a sample from the differential hybridization signals obtained in step (iv), with said typing being the allele-specific detection of a strain according to the VDG alleles present in that particular H.pylori strain, and the said virulence determinant genes being the genetic elements involved in enabling, determining, and marking of the infectivity and/or pathogenicity of said H.pylori strain. The present invention also relates to probes and primers for doing the same as well as Helicobacter pylori detecting/typing kits. The present invention also discloses novel sequences of VDG, which can be used for designing the above-mentioned primers and probes.</p> |
