Preparation of pharmaceutical grade hemoglobins by heat treatment in partially oxygenated form

摘要

During the purification of pharmaceutical grade crosslinked hemoglobin mixtures of crosslinked and uncrosslinked hemoglobin are heated in the presence of nonstoichiometric amounts of oxygen, resulting in the selective precipitation of the uncrosslinked hemoglobin. After separation of the precipitated uncrosslinked tetramers, the crosslinked hemoglobin remaining in the supernatant is so purified that a further chromatography purification step is unnecessary. This hemoglobin is highly crosslinked absolutely free of chromatography fines, and has a low methemoglobin content.