摘要 |
<p>Apparatus for disrupting cells comprises a hydraulic system (202, 203) controlled through control means to move a piston (200, 204, 210) to apply pressure to cell culture in a cylinder (211). A very fine restricted orifice (214) is provided at the end of the cylinder (211) opposite the piston (210) and the control means controls the hydraulic system such that a substantially constant pressure is exerted on the cell culture in the cylinder (211) to force the cells through the orifice (214). After the orifice (214) the cells impact on an inpact surface (224, 90, 250, 604).</p> |