| 摘要 |
Novel trehalase having the properties that it hydrolyzes alpha , alpha '-trehalose, 2,2'-dideoxy- alpha , alpha '-trehalose and 2-deoxy- alpha , alpha '-trehalose into the respective constituting sugars while it does not act on neotrehalose, lactose, maltose, celobiose and sucrose; its optimum pH is 5 to 6; its optimum temperature is 65 DEG C; it is stable against heating up to 65 DEG C; its molecular weight as measured by a gel filtration is 400,000 to 500,000 while the molecular weight of the subunit as measured by a sodium dodecylsulfate - polyacrylamide gel electrophoresis is 180,000 to 250,000; its isoelectric point as measured by an isoelectricfocusing is 2.7; and it is a glycoprotein. <IMAGE> |
