METHOD FOR DETECTING NONSENSE MUTATIONS AND FRAMESHIFT MUTATIONS

摘要

A method for conveniently detecting nonsense mutations and frameshift mutations, which is applicable even to large-sized test DNAs. This method comprises inserting, into a vector containing a promoter, an initiation codon located downstream the promoter and a reporter gene located downstream the initiation codon which is a structural gene under the regulation by the promoter and a fused polypeptide consisting of the polypeptide encoded by which and another polypeptide bonded to the N-terminus of the former polypeptide can be detected based on the function of the polypeptide encoded by the structural gene, a test nucleic acid fragment which makes the reading frame of the reporter gene to match the above-mentioned initiation codon when it is a normal type one to a site downstream the initiation codon but upstream the reporter gene; expressing the test nucleic acid fragment in the recombinant vector thus obtained and the reporter gene downstream the same in host cells; and thus examining whether or not a fused polypeptide having the function of the polypeptide encoded by the reporter gene is produced.