| 摘要 |
<p>The present invention relates to the decontamination and sterilization of pathogens, such as viruses, bacteria, and fungi present in biological materials, i.e., protein preparation, carbohydrate solutions, lipid suspensions, or cells, e.g., for administration into human or animal subjects, or for use in tissue culture systems. In particular, the invention provides a method for decontaminating a biological liquid while minimizing degradation of biological materials, such as proteins and cells. The method of the invention comprises contacting the biological liquid with sufficient reactive oxygen to deactivate microorganisms that may be present in the biological liquid by taking account of a reactive oxygen demand of the biological liquid. Critically, the maximum period of time, or effective treatment window, that the reactive oxygen is in contact with the biological liquid averts degradation of the biological material that may be present in the biological liquid. The reactive oxygen demand of the biological liquid is a function of a reactive oxygen demand of a liquid carrier and a reactive oxygen demand of any biological material that may be present in the liquid carrier. The greater the reactive oxygen demand of the biological liquid, the greater the rate of reactive oxygen transfer required to deactivate microorganisms within the effective treatment window. In specific embodiments, the invention provides for sterilization of immunoglobulin and various cells without degrading them.</p> |
