| 摘要 |
The invention concerns a method and device for treatment by complexing of a liquid medium containing one or several constituents determining the presence in said liquid medium of a plurality of ligands, which consists in: a) providing a support for plural complexing, on which are distributed a plurality of coupling sites, in which are immobilised a plurality of anti-ligands respectively different; b) contacting said liquid medium with said complexing support, whereby said ligands are paired with said anti-ligands. The invention is characterised in that the stability of each ligand/anti-ligand complex being dependent of at least one exogenous parameter, called reference parameter, conditioning said support, the coupling sites on the support are differentiated, depending on said exogenous reference parameter, having respectively different values in said sites, and the anti-ligands are distributed among the different coupling sites, depending on the predetermined optimum values of the exogenous reference parameter, determining the stability of the different ligand/anti-ligand complexes respectively. The invention is useful for sequencing nucleic acids by hybridisation.
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