SOLID PHASE IMMUNOASSAY TO DETECT INHIBITORS OF PROTEOLYTIC ENZYMES

摘要

PCT No. PCT/EP95/00867 Sec. 371 Date Sep. 23, 1996 Sec. 102(e) Date Sep. 23, 1996 PCT Filed Mar. 9, 1995 PCT Pub. No. WO95/26505 PCT Pub. Date Oct. 5, 1995Solid phase immunoassay for detecting specific inhibitors of proteolytic enzymes in biological fluids, which comprises: a) contacting a tubulin peptide covalently linked to a support with a solution containing a proteolytic activity together with a protease inhibitor, b) detecting the inhibitor activity against the selected proteases by contacting the support with a solution containing a labelled monoclonal antibody which specifically recognises the free end of the tubulin peptide linked to the support.