| 摘要 |
PROBLEM TO BE SOLVED: To detect the change of non-linear optical characteristic and to determine it by utilizing a surface secondary harmonic and sum frequency generated in a mutual action between an antibody and an antigen. SOLUTION: While a secondary optical process occurs in a structural continuous section in a boundary face and an electric field, a surface secondary harmonic signal 3 capable of being detected by virtue of change by the forming of a surface molecule layer 2 on a surface 1 has the change. When a surface non-linear coefficient of a surface layer or an orientation is changed, the characteristic of the signal 3 is changed, then it is measured. When a boundary surface incident light has cycles f1, f2 and they are not in conformity with each other, a simple extension occurs and an output of a sum frequency f1+f2≠3f is generated. If is caused by a secondary non-linear optical action of surface specificity. When a ligand stuck to an optional molecule such as an antibody or an antigen has a unique electron and a molecule having a specific transition at the surface exists, it is possible to selectively detect and determine the change of the molecule that influences the transition by utilizing the generation of the surface sum frequency.
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