一个与抗癌基因P53相作用的人类新基因P53BP3

摘要

本发明涉及一种与抗癌基因编码产物P53相作用的人体蛋白质基因P53BP3,它的cDNA由5055核苷酸组成,编码序列编码815个氨基酸的蛋白质。它在人体的正常组织和肿瘤细胞株中广泛表达。P53BP3基因定位在第12号染色体p11.2—12.1区段。

主权项

1、一种与抗癌基因编码产物P53相作用的人体蛋白质基因P53BP3，其特征在于它是通过酵母双向杂交系统获得，以下是所述人P53BP3基因的DNA核苷酸及相应的氨基酸序列：-2046 GAATTCTACTCGGTGGTTTCCCTCTGTAGGGTAGTTGGGAGCTTTGGAAGGATGTATGGG-1986 CCTGGAGGTACAAGCTCCAAGCCCTTTAGCCAACCAGTTGTTGACGCCCTGGGGCGCAGC-1926 ATCATAGGCTGTATGAGGAGAGTAGATACCGACTCTGTTCTCCAGAGCCCGGATCACCAG-1866 GCGCTCCTTCCATGTGTTCCAGGTTATGCGCTTCATGGGTCGGAAGATAGGCGGATGGTA-1806 GGAGAGAATGAGGTCTGCCTTCTTTTGCAGCACCTCCTCCATCACTTCCTCAGTCAGGTC-1746 ATTGGTCAGGAAGAGTGTATTTACAGTATGTGGTGGGCTTGGTTCCACCAGTAATCCAAC-1686 ATTGTCCCAACTCTCAGCAAACGAGAGGGATGCAAAGTCATTCAAGGAAGAAAGGAGAGC-1626 CTTCAAATCCATGAAGGAACGGGAAGAATTGCAGATCAGGGAATCTACAAACCGGACTGT-1566 CGTGGGGACTGGGCGTACGCAAGATGACAACATACAGAAATCAGGTAAAGTTCAAGTTTC-1506 TGATGTCAAAACAAAGAAAATGGTACACAGGTATACTTTAGGCCTGGATCTTTGAGGCAT-1446 CAAAACATATGGGCAAAAGAGCTGTCGTCATAGAGATCTGGGTTCAAATAGTGGCTCTGC-1386 CACTTACCAGCACACTGTAACTTAGGTAAGCCATTTAACCTCTCCAATCTTGTTTTCTCA-1326 CCTACGAAACAGAGATACTATAACCCACTTCACAGGCCATCCAGAGGAGCTAATGAATTA-1266 TGTAACCTAGCAAAGCCTGGTACATAATACACAAACGTCCATCCCATCTCCTGCCTTGCA-1206 CACCCTTGTTCTTATTCTCCCTACCCTTGCTTCAACCAACCTCCTCCCTAATCCATTTCT-1146 CTAAATATTACCTTTCCTTTTCCCAAGCCCCTCCAACTTCTTAAATAAAACTTTTCTTGA-1086 CCTCCCTTTTCAGCATCCTTCCCAAGGCTCCCCACCCCTCAGTATTCTCATAATTCTGTG-1026 TGTGCCCATTATGCTAAAGCTACTATGTAATAGGAACTAACTTCAATAAATCTCAGTATC -966 ACTAATATAAGCCAGCACTAAATGCTTACTGCATAGAATGACACTGCCCGCCTCCCCTGA -906 TTGCAACAGAATTTGGAGTTGGGGTGGTATATACTGGCATTACAATGTGCTTTACCAAAA -846 AACAGAATAATGTGGCGGGGCGCCTGTAATCCCGCGGCCGCGTCGACATAGGGCCTTTCC -786 GCCTGCGGGCCCAGTGAGTCGACACGGTGGGGGCCCGCGATCCCCGGGACTTACCACTGA -726 ATAAATCCAGTCCAGCGTGCGACGACTTACTGGCTTCATGGGGTCGCAGCCGCCGCTGGG -666 GTCTCCGCTGTCTCGCGAGGAGGGTGAAGCGCCCCGCCTGCTCCCGCTTCGGAGGGTAGG -606 CGGAGAAGTCGCCGGGTACGCCTTCGCGGATCCTGCCGACACCGACCTAGCTTTCTGGGC -546 TGCCGGGAGCTCGCGGCGAGCGCCCCAGCCAGGCCTGCGCCGGCATCCTCCGAGATAATG -486 GCATCAGCTGCTAAGGAATTTAAAATGGACAACTTTTCACCTAAAGCTGGCACTAGCAAA -426 TTGCAACAGACAGTACCAGCTGATGCATCTCCTGATTCTAAGTGTCCTATATGCTTGGAT -366 AGATTTGATAATGTGTCTTACTTAGATCGCTGCTTACATAAGTTCTGAAAACGCTGTGTA -306 CAGGAGTGGTCAAAAAACAAAGCTGAATGCCCACTATGTAAACAGCCCTTTGATTCTATT -246 TTCCATTCTGTGAGGGCAGAAGATGACTTCAAGGAGTATGTCCTAAGGCCTTCGTATAAT (后续)-186 GGTTCTTTTGTCACCCCTGATCGACGATTTCGCTACCGTACAACTCTGACAAGGGAACGA-126 AATGCTTCTGTGTATTCACCTAGTGGTCCTGTGAACAGAAGAACAACAACTCCACCGGAT -66 AGTGGAGTACTGTTTGAAGGGTTAGGCATTTCAACAAGACCTAGAGATGTTGAAATTCCT -6 CAGTTTATGAGACAGATTGCAGTAAGGAGGCCAACTACGGCAGATGAAAGATCTTTGCGG M R Q I A V R R P T T A D E R S L R 55 AAAATTCAAGAACAAGATATTATTAATTTTAGACGAACTCTTTATCGTGCTGGTGCTCGA K I Q E Q D I I N F R R T L Y R A G A R 115 GTTAGAAATATTGAAGATGGTGGCCGCTACAGGGATATTTCAGCTGAATTTTTCCGTAGA V R N I E D G G R Y R D I S A E F F R R 175 AATCCAGCTTGCCTTCACAGATTAGTCCCCTGGTTAAAACGTGAACTTACAGTTCTTTTT N P A C L H R L V P W L K R E L T V L F 235 GGAGCTCATGGATCTTTAGTGAATATTGTCCAGCATATTATCATGAGTAATGTTACTCGC G A H G S L V N I V Q H I I M S N V T R 295 TATGACTTGGAGAGTCAGGCATTTGTGTCTGATTTAAGACCATTTTTACTTAATCGAACT Y D L E S Q A F V S D L R P F L L N R T 355 GAGCATTTTATACATGAATTTATCAGTTTTGCCCGATCTCCTTTTAACATGGCAGCCTTT E H F I H E F I S F A R S P F N M A A F 415 GACCAGCATGCCAATTATGATTGCCCTGCTCCTTCATACGAAGAAGGCAGCCATTCTGAT D Q H A N Y D C P A P S Y E E G S H S D 475 TCTTCAGTCATAACAATATCTCCAGATGAGGCTGAGACCCAAGAGCTGGATATTAATGTA S S V I T I S P D E A E T Q E L D I N V 535 GCCACTGTTAGTCAGGCACCATGGGATGATGAAACTCCAGGACCATCTTACTCAAGCTCA A T V S Q A P W D D E T P G P S Y S S S 595 GAGCAGGTACACGTTACTATGTCTTCTCTTTTAAATACTTCTGACAGTTCAGATGAAGAA E Q V H V T M S S L L N T S D S S D E E 655 CTTGTCACAGGAGGAGCCACGTCTCAGATACAAGGAGTACAAACCAATGACGACCTAAAT L V T G G A T S Q I Q G V Q T N D D L N 715 AATGACAGTGATGATTCTTCAGATAATTGTGTCATTGTTGGGTTTGTTAAACCACTAGCT N D S D D S S D N C V I V G F V K P L A 775 GAGAGGACCCCAGAACTTGTTGAACTGTCCTCTGATTCTGAGGACTTAGGTTCTTATGAG E R T P E L V E L S S D S E D L G S Y E 835 AAAATGGAGACAGTGAAGACACAAGAACAGGAGCAATCTTACAGTTCTGGTGATAGCGAT K M E T V K T Q E Q E Q S Y S S G D S D 895 GTTAGTAGATGCTCATCTCCACACTCTGTCCTTGGAAAGGATGAACAAATAAATAAAGGT V S R C S S P H S V L G K D E Q I N K G 955 CATTGTGATTCTAGTACAAGAATCAAATCAAAGAAGGAAGAGAAACGATCTACATCATTG H C D S S T R I K S K K E E K R S T S L1015 TCATCTCCCAGAAACCTGAACTCATCTGTAAGAGGAGACAGAGTATATTCTCCATATAAC S S P R N L N S S V R G D R V Y S P Y N1075 CATAGACACAGAAAGAGGGGAAGATCAAGAAGTTCAGATTCACGTTCTCAGAGTAGAAGT H R H R K R G R S R S S D S R S Q S R S1135 GGGCATGATCAGAAGAATCATAGAAAGCATCATGGGAAGAAAAGAATGAAAAGTAAACGA G H D Q K N H R K H H G K K R M K S K R1195 TCCAGAAGCAGGGAAAGTAGCAGACCTAGAGGGAGAAGAGACAAAAAGAGATCAAGAACT S R S R E S S R P R G R R D K K R S R T1255 AGAGATAGCAGTTGGTCCAGAAGAAGCCAAACTCTGTCTCTAAGTAGTGAAAGCACAAGC R D S S W S R R S Q T L S L S S E S T S1315 AGATCAAGGTCTCGTAGCAGTGATCATGGTAAAAGAAGATCACGGAGCAGAAATAGAGAT R S R S R S S D H G K R R S R S R N R D1375 CGTTATTATTTAAGAAATAATTATGGAAGCAGATACAAATGGGAGTATACTTATTACAGT R Y Y L R N N Y G S R Y K W E Y T Y Y S (后续)1435 AGAAACAAGGACAGGGATGGGTACGAATCATCTTACAGGAGGAGGACTCTGTCCAGAGCT R N K D R D G Y E S S Y R R R T L S R A1495 CATTATTCTAGACAGTCTTCAAGTCCAGAATTTAGAGTTCAGTCCTTTTCTGAAAGAACA H Y S R Q S S S P E F R V Q S F S E R T1555 AATGCTAGGAAAAAAAATAATCACAGTGAGAGGAAGTATTACTACTATGAAAGGCACAGA N A R K K N N H S E R K Y Y Y Y E R H R1615 TCAAGGAGCCTGTCTAGTAACAGATCAAGGACTGCATCTACCGGGACTGACCGGGTGAGA S R S L S S N R S R T A S T G T D R V R1675 AATGAAAAGCCTGGAGGGAAACGAAAATACAAAACACGGCATTTGGAGGGTACTAACGAA N E K P G G K R K Y K T R H L E G T N E1735 GTGGCTCAGCCATCTCGTGAATTTGCTTCTAAAGCAAAGGACAGTCATTACCAAAAATCT V A Q P S R E F A S K A K D S H Y Q K S1795 TCATCAAAATTGGATGGAAACTACAAAAATGAGAGTGATACCTTTTCAGACAGCCGATCA S S K L D G N Y K N E S D T F S D S R S1855 TCAGACAGAGAGACAAAACACAAAAGGAGAAAAAGGAAGACCCGGAGCCTAAGTGTAGAG S D R E T K H K R R K R K T R S L S V E1915 ATAGTTTATGAAGGAAAAGCTACTGATACAACTAAACACCATAAAAAGAAAAAGAAGAAA I V Y E G K A T D T T K H H K K K K K K1975 CATAAGAAGAAGCATAAGAAACACCATGGAGATAATGCTTCACGTTCCCCAGTTGTAATT H K K K H K K H H G D N A S R S P V V I2035 ACCATTGACAGTGACAGTGATAAGGATTCTGAAGTAAAGGAGGATACAGAATGTGACAAT T I D S D S D K D S E V K E D T E C D N2095 AGTGGTCCTCAAGACCCTCTACAAAATGAGTTTTTGGCTCCTTCCTTGGAACCATTTGAA S G P Q D P L Q N E F L A P S L E P F E2155 ACTAAAGATGTAGTTACAATAGAAGCTGAATTTGGTGTGCTGGACAAGGAATGTGATATT T K D V V T I E A E F G V L D K E C D I2215 GCCACACTTAGTAACAACTTGAATAATGCCAACAAAACTGTAGATAATATTCCACCTCTG A T L S N N L N N A N K T V D N I P P L2275 GCAGCTTCAGTTGAACAAACTCTCGATGTAAGAGAAGAGAGCACCTTTGTTTCTGATTTG A A S V E Q T L D V R E E S T F V S D L2335 GAGAACCAGCCCAGTAACATTGTGTCTCTTCAAACTGAGCCATCAAGGCAATTGCCATCG E N Q P S N I V S L Q T E P S R Q L P S2395 CCACGGACATCATTAATGTCAGTATGTCTTGGTAGAGACTGTGATATGTCTTAAAACTGC P R T S L M S V C L G R D C D M S *2455 CAAAGCATTTCATTGAGAATTATGATGTTATAAAAAGGAAAAAGGAAGAATGTCGTCTAC2515 TGCAGTCTATTTAAAGATGACATTTGGTGAAAACTCTCTTCCTCCTTACAATATTTTAAA2575 TGATTTTTTTTTTGGTGTTAATTTGTAAAAATCATTATTTGTTCAAAATGTATGTCCCAC2635 CCTCAAAGATATGCACTTTTAAGTGAAGAAAATGATACTGCTAGCAGCTTATTTAAAACT2695 TGGGGTCCTTTTTAAATAAGAAAAATTATATAAATTTTAGAAGTTATTTCATAAAGCCAT2755 ACGGTATTGACATATTTTTAAGGTAGTCAATGAGTATTTTTGAATTTTTTTTTTTTGAGA2815 GTTATTCTGGAAATGTGTTATAAGCTAGGAGAATCCCTTTGGACAGTCTTTATTTTTCTT2875 CTTAAAAATTTATATGATTCAAAACCATTTCTTCAGGTTAAATTGAGGCATTTTAATCTG2935 CACAGTTTATCTTCTGCCAAAATAAAAATTTACTATTTCCTTTATATACTTGTTTATCTG2995 GACTGCCAGTAAAAC