发明名称 |
IMPROVED PRODUCTION OF RIBOFLAVIN |
摘要 |
PROBLEM TO BE SOLVED: To secrete and produce a riboflavin useful for preparing a food or a composition for a feed by culturing a specific recombinant bacterium transformed with three or four vectors. SOLUTION: A recombinant bacterium transformed with three or four vectors or DNA sequences is grown under proper growth conditions. Two of the vectors or DNA sequences respectively comprises a DNA sequence capable of coding riboflavin synthase activities of Bacillus subtilis (or a substantially homologous DNA sequence) and one or more transcription elements and further the third and/or the fourth vector or DNA sequence comprises a DNA sequence coding a ribA gene product of the Bacillus subtilis (or the substantially homologous DNA sequence) and optionally a transcription element. One or plural copies of the vectors are integrated into three of four different sites of a chromosome of a bacterium with the transcription elements. |
申请公布号 |
JPH1084978(A) |
申请公布日期 |
1998.04.07 |
申请号 |
JP19970184843 |
申请日期 |
1997.07.10 |
申请人 |
F HOFFMANN LA ROCHE AG |
发明人 |
HOHMANN HANS-PETER;HUEMBELIN MARKUS;VAN LOON ADOLPHUS;SCHURTER WALTER |
分类号 |
A23K1/16;A23L1/30;C07H21/04;C12N1/21;C12N15/00;C12N15/09;C12N15/52;C12P25/00;C12R1/125 |
主分类号 |
A23K1/16 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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