MASS CULTURE METHOD FOR CARROT ADVENTIOUS EMBRYO

摘要

PROBLEM TO BE SOLVED: To enable efficient mass culture of the carrot adventive embryos by culturing carrot adventive embryos while controlling the oxygen supply rate, ventilation rate and value of physical stress applied to the adventive embryos so as to meet specified culture conditions respectively. SOLUTION: (1) An oxygen transfer volume coefficient (KLa ) that is a quantitative indicator of the oxygen supply rate and shows the oxygen transfer rate from a gas phase to a liquid phase and is defined by the formula I (wherein: dC/di is the oxygen transfer rate (mgO2 /L.hr); C is the dissolved oxygen concn. (mgO2 /l); C' is the saturated dissolved oxygen concn. and (t) is time (hr)); (2) a ventilatory ethanol driving-out rate (VEt ) that is a-quantitative indicator of the ventilation rate and shows the capability of driving out accumulated gases in a culture medium by ventilation and the reduction in the amount of ethanol at the time of adding ethanol to the culture medium and shaking the resulting medium under the same conditions as those under which the adventive embryos are to be introduced into the culture medium and is defined by the formula II (wherein: Etinit is the initial ethanol concn. (mM) in the medium at the beginning of the shaking; Etfin is the final ethanol concn. in the medium at the end of the shaking; and T is the shaking time (hr)); (3) a material dissolution rate coefficient (K) that is a quantitative indicator of the physical stress value and is measured in aβ-naphthol-water system and is defined by the formula III (wherein dS/dt is the material dissolution rate (mg/ml.sec); S is the concn. of solid dissolved in time (t)(sec); S' is the saturated concn. of the solid; A is the total surface area (cm<2> ) of solid particles; and V is the liquid volume (ml) of the resulting solution); and (4) culture conditions represented by the formula IV.