CONTROLLED EXCISION

摘要

Integrating a gene in a bacterial chromosome and controlling excision of the gene from the chromosome in response to an external stimulus, using DNA comprising: a) a vector that is incapable of replicating autonomously in the host and that includes the gene (or a site for the insertion of the gene) and an attachment site corresponding to an attachment site on the host chromosome; b) DNA capable of causing the integration of vector a) in the bacterial chromosome and DNA capable of causing the excision of that vector therefrom; and c) DNA capable of effecting control of excision of vector a) from the bacterial chromosome in response to an externally controlled stimulus.