摘要 |
PROBLEM TO BE SOLVED: To easily evaluate the in vivo antioxidizing power of a test agent after its oral administration, by applying a photoinducible radical-forming substance to the skin of a test animal orally administering the test agent followed by photoirradiation and then measuring the quantity of radicals formed. SOLUTION: The skin (pref. the skin of the back) of a test animal (pref. a hairless mouse) orally administered with a test agent is coated with a photoinducible radical-forming substance (pref. anthralin) followed by photoirradiation to form radicals, whose quantity is then measured. The radiation for the irradiation is pref. ultraviolet rays. A preferable method of the measurement is such one that the skin of the test animal after subjected to the irradiation is peeled off and then put into a tissue test tube for ESR(electron spin resonance) measurement to determine the radical quantity by the use of X-band ESR. On the basis of the radical intensity of a substance which has been known to have no antioxidizing effect, the evaluation of the antioxidizing power of the test agent can be accomplished through the lowering of its radical intensity. |