Gas permeable pouches for growing cultures

摘要

Cultures, such as bacterial, fungal, and mammalian cells and viruses of the above may be grown without agitation in culture pouch vessels having gas permeable surfaces. The culture pouches may be constructed of gas permeable silicone rubber and may include a sealable filling port and integral cuvette for measuring bacteria density. The culture pouches may be supplied with sterile dry culture medium, and culture growth may be initiated in the pouches by adding cells and sterile water.