Method of inhibiting angiogenesis

摘要

The present invention is directed to methods of inhibiting angiogenesis and methods of inhibiting endothelial cell proliferation by the administration an effective amount of angiostatin protein. The angiostatin protein is an endothelial inhibitor and is isolated from the blood of urine that is eluted as a single peak from C4-reverse phase high performance liquid chromatography. Angiostatin is a molecule comprising a protein having a molecular weight of between approximately 38 kilodaltons and 45 kilodaltons as determined by reducing polyacrylamide gel electrophoresis and having an amino acid sequence substantially similar to that of a murine plasminogen fragment beginning at amino acid number 9.8 of a murine plasminogen molecule.