A random peptide library constructed by transforming host cells with a collection of recombinant vectors that encode a fusion protein comprised of a DNA binding protein and a random peptide and also encode a binding site for the DNA binding protein can be used to screen for novel ligands. The screening method results in the formation of a complex comprising the fusion protein bound to a receptor through the random peptide ligand and to the recombinant DNA vector through the DNA binding protein.
申请公布号
US5733731(A)
申请公布日期
1998.03.31
申请号
US19950548540
申请日期
1995.10.26
申请人
AFFYMAX TECHNOLOGIES N.V.
发明人
SCHATZ, PETER J.;CULL, MILLARD G.;MILLER, JEFF F.;STEMMER, WILLEM PETER CHRISTIAAN;GATES, CHRISTIAN M.