发明名称 |
Cytotoxicity-based genetic selection system (TOXSEL) |
摘要 |
The invention provides a cytotoxicity-based genetic selection (TOXSEL) method and related testing kit for the identification and positive selection of molecules or mutations that are capable of disrupting a specific protein-protein interaction. The invention enables positive selection of molecules disruptive of specific protein-protein interactions by virtue of the presence in the TOXSEL system of a toxin reporter gene. A disrupted protein-protein interaction precludes expression of the toxin reporter gene and, consequently, allows survival of the host cell. TOXSEL technology enables large-scale screening for drugs or small molecules capable of disrupting specific protein-protein interactions critical in processes such as cellular signalling, carcinogenesis, etc. The positive selection of molecules or mutations capable of interfering with specific protein-protein interactions permits the identification of amino acid residues or motifs critical in the protein-protein interactions and permits the design of drugs for therapeutic or diagnostic applications.
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申请公布号 |
US5733726(A) |
申请公布日期 |
1998.03.31 |
申请号 |
US19950472238 |
申请日期 |
1995.06.07 |
申请人 |
EMORY UNIVERSITY;PRESIDENT AND FELLOWS OF HARVARD COLLEGE |
发明人 |
FU, HAIAN;COLLIER, R. JOHN;DINGLEDINE, RAYMOND |
分类号 |
C12N15/09;A61K45/00;C12N1/19;C12N5/10;C12N15/10;C12P21/02;C12Q1/02;C12Q1/68;G01N33/50;(IPC1-7):C12Q1/68;C12N15/79 |
主分类号 |
C12N15/09 |
代理机构 |
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