发明名称 |
Compositions and methods for detecting target nucleic acid sequences utilizing adjacent sequence-enzyme molecules |
摘要 |
Briefly stated, the present invention provides novel compositions and methods for detecting target nucleic acid sequences utilizing adjacent sequence-enzyme molecules. Within one aspect of the present invention, oligonucleotide-enzyme fusion molecules are provided, comprising an enzyme capable of cleaving scissile linkages and an oligonucleotide having the structure (NA1)x-Sz-(NA2)y wherein NA1 and NA2 are nucleic acid sequences, S is a scissile nucleic acid linkage, x, y and z are integers from 1 to 100 and n is an integer from 1 to 10.
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申请公布号 |
US5731146(A) |
申请公布日期 |
1998.03.24 |
申请号 |
US19950474624 |
申请日期 |
1995.06.06 |
申请人 |
ID BIOMEDICAL CORPORATION |
发明人 |
DUCK, PETER D.;BEKKAOUI, FAOUZI;CROSBY, WILLIAM L.;TULLIS, RICHARD H. |
分类号 |
C12N15/09;C07K14/00;C12N9/00;C12N9/22;C12Q1/34;C12Q1/68;(IPC1-7):C12Q1/68;C07H21/04;C07H21/02 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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