发明名称 |
A PROCESS FOR PURIFYING APOLIPOPROTEINS AND A COMPOSITION FOR USE IN THE PROCESS |
摘要 |
The present invention relates to a composition for use in purification of apolipoprotein A (ApoA) or apolipoprotein E (ApoE), said composition comprising a first and a second polymeric material, wherein the first and second polymeric materials are immiscible in the primary aqueous solution, and wherein the second polymeric material is amphiphilic and water soluble. The invention further relates to a process for purifying ApoA or ApoE, or variants or mixtures thereof, by first mixing ApoA or ApoE, the composition containing a first and second polymeric material and water. The resulting primary aqueous solution is maintained for a period of time sufficient for essentially separating the phases formed, and removing the phase containing the second polymeric material and the main portion of ApoA or ApoE. Subsequently, the second polymeric material is separated from ApoA or ApoE. The thus produced ApoA or ApoE can be used for the manufacture of a medicament in the treatment of atherosclerosis and cardiovascular diseases, sepsis orperipheral atherosclerosis as well as in a method for treatment of atherosclerosis and cardiovascular diseases, sepsis or peripheral atherosclerosis when administered in a therapeutically effective amount.
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申请公布号 |
WO9811140(A1) |
申请公布日期 |
1998.03.19 |
申请号 |
WO1997SE01501 |
申请日期 |
1997.09.08 |
申请人 |
PHARMACIA & UPJOHN AB;AGELAND, HANS;NYSTROEM LENA;PERSSON, JOSEFINE;TJERNELD, FOLKE |
发明人 |
AGELAND, HANS;NYSTROEM LENA;PERSSON, JOSEFINE;TJERNELD, FOLKE |
分类号 |
C12N1/14;A61K38/00;A61P9/00;A61P9/10;C07K14/775;C12N1/20;(IPC1-7):C07K14/775;C07K1/14;C07K1/20 |
主分类号 |
C12N1/14 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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