| 摘要 |
<p>A medium and method for culturing lymphocytes are provided for determining intracellular concentration of glutathione or cysteine in human lymphocytes to provide biochemical analysis of an individual's capability of dealing with oxidative stress. The medium is a buffered serum-free solution having a pH of from about 6.8 to 7.6 and containing a carbohydrate which is glucose or a compound capable of producing glucose in lymphocytes, pantothenic acid, choline or a substance capable of producing choline in lymphocytes, inorganic ions including chloride, phosphate, calcium, magnesium, potassium, sodium and iron, L-Buthionine-[S.R.]-Sulfoximine, deionized water and a mitogen to stimulate lymphocytes. When determining cysteine concentration, the medium additionally contains N-Acetyl-L Cysteine and Cumene Hydroperoxide. The method is carried out by inoculating the culture medium with lymphocytes from an individual, incubating the lymphocytes in the medium and comparing the response of the lymphocytes with an average response of lymphocytes from a control group of individuals.</p> |
