METHOD AND KIT FOR THE DIAGNOSIS OF BLACKCURRANT REVERSION DISEASE

摘要

The present invention relates to a method for diagnosing blackcurrant reversion disease in plants, especially in blackcurrant (Ribes) plants. According to the invention a reverse transcriptase-PCR protocol optionally in combination with immunocapture, is used to detect a virus causing blackcurrant reversion disease, blackcurrant reversion virus, BRV. The invention also concerns a test kit for use in the method. In the work leading to the invention, the BRV RNA was partially characterized. This enabled primers to be constructed from the viral nucleotide sequence so determined for use in the method and the kit of the invention.