| 摘要 |
PROBLEM TO BE SOLVED: To provide a method for rapid identification and determination of effects of an antimicrobial agent. SOLUTION: This method for test comprises the following steps (a) to (d): (a) incubating a specimen of a microorganism in a culture medium containing an antimicrobial agent at a continuously diluted concentration in a short time and providing the different total numbers of microorganisms, (b) amplifying the total different numbers of microorganisms according to an in vitro microbial DNA replication, (c) adding a reporter molecule, specific for a double-stranded DNA (dsDNA) and capable of forming a complex therewith to the resultant DNA amplification products and (d) observing the presence or absence of a signal release from the reporter molecule-dsDNA complex.
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