A PRECISE EVALUATION METHOD OF SHEATH BLIGHT RESISTANCE USING LIQUID CULTURED MYCELIA

摘要

A method for evaluating sheath blight resistance is provided to obtain an objective evaluation result conveniently regarding the sheath blight resistance, raise the inoculation efficiency of pathogenic bacteria of the sheath blight and improve the resistance evaluation method, thereby contributing to breed a species with resistance to the sheath blight. A method for evaluating sheath blight resistance comprises the steps of: (a) after inoculating a liquid culture mycelia of Rhizoctonia solani into rice, sealing the inoculation portion tightly using a shielding film such as aluminum foil for maintaining moisture required for vitality of the mycelia; and (b) after cultivating the mycelia inoculated rice until disease is progressed into branches and leaves, evaluating the resistance degree, wherein the resistance degree evaluation is performed by evaluating the attack degree of all leaves sheaths corresponding to the inoculated stem with 0 to 9 steps and calculating a virus infection rate of the inoculated stem in accordance with a following calculating formula of the virus infection rate being equal to (9n9+7n7+5n5+3n3+1n1+0n0)/9N Î 100(wherein each of the n0 to n9 is a number of leaves sheaths corresponding to each of the attack degree evaluated at the mycelia inoculated stem, and the N is the total leaves sheaths of the mycelia inoculated stem).