发明名称 |
E2f ubiquitination domain, and assays for inhibitors of e2f ubiquitination |
摘要 |
The transcription factor E2F contains a ubiquitination domain. Assays for inhibitors and enhancers of the domain are provided. An assay method for an inhibitor of transcription factor E2F ubiquitin-mediated degradation comprises a) bringing a polypeptide which contains a domain which renders E2F a substrate for ubiquitination into contact with a candidate inhibitor; and b) determining whether or not the candidate inibitor is capable of reducing ubiquitination of the polypeptide. An assay method for an enhancer of transcription factor E2F ubiquitin-mediated degradation may comprise a) bringing a polypeptide which contains a domain which renders E2F a substrate for ubiquitination into contact with a candidate enhancer; and b) determining whether or not the candidate enhancer is capable of enhancing ubiquitination of said polypeptide. |
申请公布号 |
AU3951097(A) |
申请公布日期 |
1998.03.06 |
申请号 |
AU19970039510 |
申请日期 |
1997.08.22 |
申请人 |
VERENIGING HET NEDERLANDS KANKER INSTITUUT;PROLIFIX LIMITED |
发明人 |
RENE BERNARDS |
分类号 |
G01N33/50;C07K14/47;C12N15/09;C12N15/12;C12Q1/02;G01N33/15 |
主分类号 |
G01N33/50 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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