| 摘要 |
The present invention provides methods and kits for detection, and optionally quantitation, of multiple nucleic acid sequences. The assays of the invention, as illustrated in the figure, employ a plurality of species of capture probes, each species comprising a bacteriophage covalently or non-covalently linked to an oligonucleotide complementary to one analyte, wherein the bacteriophage of each species is capable of generating a distinctive signal when plated on an indicator medium. The oligonucleotide moieties of each capture probe contain a sufficient number of contiguous ribonucleotides to confer sensitivity to a ribonuclease specific for RNA/DNA or RNA/RNA duplexes. In the method of the invention, the capture probe species are combined with the fluid sample, which has been processed to releases ingle-stranded nucleic acids. Hybridization is allowed to occur, thereby generating DNA/RNA or RNA/RNA duplexes. By adding a nucleolytic enzyme capable of cleaving DNA-RNA duplexes, bacteriophage are released for detection of the different signals on an indicator medium. The kit of the invention provides components which allow the method of the invention to be performed. |
