| 摘要 |
PROBLEM TO BE SOLVED: To efficiently carry out the search and isolation of an active gene, provide a causative gene from a genomic region of useful character expression, genetic diseases, etc., and conduct the gene therapy by determining the base sequence such as the upstream side, etc., of a restriction enzyme site contained in a rear cutter restriction map. SOLUTION: A rare cutter restriction map such as NotI, EagI, BssHII, SacII, MluI, NruI or SaII is prepared on the basis of a physical map of a specific region in a chromosome. A base sequence contained in the restriction map and on the upstream and downstream side of two or more restriction enzyme sites contained in a DNA region of <=1kb is determined to search for and isolate an active gene, substantially containing a base sequence represented by the formula and causing Down's syndrome. |
