Systems and devices for isothermal biochemical reactions and/or analysis

摘要

An isothermal reaction and analysis system may include a receiver to receive sample holders, a thermal control subsystem to control a temperature of the receiver, an excitation subsystem, a detection subsystem and an analysis subsystem. Excitation sources and/or detectors are positioned to enhance data collection. Sample holders may include filters, selectively blocking and passing wavelengths or bands of electromagnetic radiation.

主权项

1. A system to support isothermal nucleic acid amplification reactions in a plurality of samples, the system comprising:
a receiver having a plurality of receptacles formed therein, each of the receptacles sized to at least partially receive and support a respective one of a plurality of sample holders wherein the receiver has a large thermal mass relative to a respective thermal mass of the samples; an excitation subsystem including at least a first plurality of excitation sources, each of the excitation sources having a respective axis of emission and positioned to direct electromagnetic energy toward at least a portion of a respective one of the sample holders received in a respective one of the receptacles of the receiver, the excitation subsystem operable to emit electromagnetic energy with a first wavelength sufficient to excite fluorescence in each of the plurality of samples contained in respective ones of the plurality of the sample holders while the sample holders are received and supported by the receiver; a detection subsystem that includes a plurality of detectors, each of the detectors having a respective line of sight and positioned to detect fluorescence emission of electromagnetic energy at a wavelength that is different from the first wavelength and which is emitted by a respective sample contained in a respective one of the sample holders that are received and supported by the receiver, each of the detectors positioned such that the respective line of sight of the detector and the respective axis of emission of the respective excitation source are arranged at an angle to reduce the detection of emission directly from the excitation sources; and a thermal control subsystem operable to at least approximately maintain an at least approximately uniform temperature of the receiver at least approximately and said temperature constant for a period of time sufficient to perform an isothermal nucleic acid amplification on the samples.