发明名称 |
Surface modification of polymers with self-assembled monolayers that promote adhesion, outgrowth and differentiation of biological cells |
摘要 |
A process for forming spatially oriented neo-vascular capillaries comprising: (a) providing a combination ultra-thin film (UTF) pattern of at least one cell adhesion promoter and at least one cell adhesion inhibitor wherein the cell adhesion promoters have a linewidth of between about 50-490 mu m; (b) seeding the combination UTF pattern with endothelial cells at an initial seeding cell density; (c) adding a first medium for incubating the seeded endothelial cells until the endothelial cells are grown to confluency; (d) replacing the first medium with a second medium at confluency; and (e) allowing the endothelial cells to differentiate into spatially oriented neo-vascular capillaries. |
申请公布号 |
US5721131(A) |
申请公布日期 |
1998.02.24 |
申请号 |
US19940234034 |
申请日期 |
1994.04.28 |
申请人 |
UNITED STATES OF AMERICA AS REPRESENTED BY THE SECRETARY OF THE NAVY |
发明人 |
RUDOLPH, ALAN S.;CHU, CHIH-CHANG;STENGER, DAVID A.;SPARGO, BARRY J.;GEORGER, JACQUE |
分类号 |
B05D1/18;C09D4/00;C12N5/00;C23C18/16;C40B60/14;G03F7/004;G06N3/00;G06N99/00;H01L21/288;H05K3/06;(IPC1-7):C12N5/00 |
主分类号 |
B05D1/18 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|