发明名称 |
Recombinant human cytomegalovirus vaccine |
摘要 |
Infection of human fibroblast cells with human cytomegalovirus (HCMV) causes down regulation of cell surface expression of MHC class I. The present invention is directed to a mutant with a 9-kb deletion in the S component of the HCMV genome (including open reading frames IRS1-US9 and US11) which failed to down regulate class I heavy chains. By examining the phenotypes of mutants with smaller deletions with this portion of the HCMV genome, a 7-kb region containing at least 9 open reading frames was shown to contain the genes required for reduction in heavy chain expression. Furthermore, it was determined that two subregions (A and B) of the 7-kb region each contained genes which were sufficient to cause heavy chain down regulation. In subregion B, the US11 gene product is involved. It encodes a endoglycosidase H-sensitive glycoprotein which is intracytoplasmic, similar to the adenovirus type 2 E3-19K glycoprotein which inhibits surface expression of class I heavy chains.
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申请公布号 |
US5720957(A) |
申请公布日期 |
1998.02.24 |
申请号 |
US19950459586 |
申请日期 |
1995.06.02 |
申请人 |
AMERICAN CYANAMID COMPANY |
发明人 |
JONES, THOMAS R.;CAMPBELL, ANN E. |
分类号 |
C12N15/00;A61K31/70;A61K35/76;A61K38/00;A61K39/245;A61K48/00;A61P31/12;C07K14/045;C12N7/00;C12N7/01;C12N7/04;C12N15/01;C12N15/09;C12N15/34;C12N15/38;C12N15/86;C12N15/861;C12R1/92;(IPC1-7):A61K39/245 |
主分类号 |
C12N15/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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