发明名称 |
METHOD OF OBTAINING ACYLOINS, PYRUVATE DECARBOXYLASE SUITABLE THEREFOR AS WELL AS PROCESS FOR PREPARING THE PYRUVATE DECARBOXYLASE AND DNA SEQUENCE FOR PDC GENE ENCODING THEREOF |
摘要 |
A process for obtaining a pyruvate decarboxylase by isolation from a producer organism. The pyruvate decarboxylase is capable of forming (R)-(-)-phenylacetylcarbinole (I) in >/=95% enantiomer unit with a product ratio of (I) to 2-hydoxypropiophenone of >/=95%. In addition, the pyruvate decarboxylase has a specific activity with regard to phenylacetylcarbinole formation of >1U/mg. It is the aim of the invention to obtain a pyruvate decarboxylase with improved synthesis capacity concerning the formation of (R)-(-)-phenylacetylcarbinole. The process of the invention developed for this purpose is characterized in that use is made of a producing organism with a gene coding for pyruvate decarboxylase from Zymomonas mobilis, in the DNA sequence of which the tryptophane radical coding codon TGG is replaced at position 1174-1176 by a codon which codes for an amino acid radical with a reduced volume ratio. |
申请公布号 |
CZ9703691(A3) |
申请公布日期 |
1998.02.18 |
申请号 |
CZ19970003691 |
申请日期 |
1996.05.22 |
申请人 |
FORSCHUNGSZENTRUM JULICH GMBH;BRUHN HEIKE;POHL MARTINA;MESCH KATRIN;KULA MARIA REGINA |
发明人 |
BRUHN HEIKE;POHL MARTINA;KULA MARIA REGINA;MESCH KATRIN |
分类号 |
C12N15/09;C12N9/02;C12N9/88;C12N15/00;C12N15/60;C12P7/26;C12P7/42;C12R1/19;(IPC1-7):C12N15/60;C07C49/245 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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