| 摘要 |
PROBLEM TO BE SOLVED: To obtain a new transformant which is transformed with a recombinant plasmid containing a gene coding a functional polypeptide having a specific amino acid sequence, useful for producing a functional polypeptide, especially a human fibronectin, etc. SOLUTION: This new transformant is transformed with a recombinant plasmid containing a gene coding a functional polypeptide having an amino acid sequence of the formula and is useful for producing the functional polypeptide participating especially in physiological actions of adhesion, spreading, movement, differentiation, proliferation, phagocytosis, etc., of cell and taking an important role in construction and restoration of tissue, blood coagulation, biophylaxis, etc. The transformant is obtained by cloning a cDNA coding a cell adherent domain of a human fibronectin, incorporating the obtained cDNA fragment into a secretion type vector, inserting the obtained recombinant vector into a host such as Escherichia coli, etc., and transforming. |
